Dibrugarh University| B.SC - (CBCS)| BOTANY - (Honours/ Non-Honours) (DSE - 1)| Question Paper - (Nov/ Dec) - 2022| 5th Semester
5 SEM TDC DSE BOT (CBCS) 1 (H/NH)
2022
(Nov/ Dec)
BOTANY
(Discipline Specific Elective)
(For Honours & Non-Honours)
Paper: DSE-1
(Analytical Techniques in Plant
Science)
Full Marks: 53
Pass Marks: 21
Time: 3 hours
The figures in the margin indicate
full marks for the questions
1. (a) Choose the correct answer of the following: 1x3=3
(i) If the
eyepiece magnification of light microscope is x10 and objective is x40, then
the overall magnification is x40/ x10/ x400/ x4.
(ii) Rf
value is used in spectrophotometry/ chromatography/ electrophoresis/ None of these.
(iii) When n
is an odd number, then median is defined as middle value/ sum of the values/
median of two middle values/ most repeated value.
(b) Fill in
the blanks: 1x2=2
(i) In _______chromatography,
the stationary phase is held in a narrow tube and the mobile phase is forced
through it under high pressure.
(ii) The
technique of electrophoresis was developed by _______.
2. Write short notes on (any three): 4x3=12
(a) Cell
fractionation
(b) Density-gradient
centrifugation
(c) Page
(d) Chromosome
painting
3. Explain the different techniques used in sample
preparation for election microscopy. Describe how freeze fracture technique
helps in understanding the membrane structure. How is freeze fracture different
from freeze-etching? 4+5+3=12
Or
What is gel
electrophoresis? Describe the method of separation of nucleic acid by gel
electrophoresis. 3+9=12
4. Describe the working principle and instrumentation of a spectrophotometer.
Also discuss its role in biological research.3+4+5=12
Or
What is ion
exchange chromatography? Describe its principle, instrumentation and uses.
3+3+3+3=12
5. What is standard error? Calculate the mean, mode and median
from the following data: 3+3+3+3=12
|
CI |
0-10 |
10-20 |
20-30 |
30-40 |
40-50 |
|
Frequency |
5 |
8 |
15 |
16 |
6 |
Or
Discuss the
necessity of the application of statistical sciences in biological sciences
with example. 8+4=12
***
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